Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The effects of vincristine on WRL68 cells and cell viability were assessed using the CCK-8 method. Cells were treated with different concentrations of vincristine (0.00, 0.01, 0.10, 1.00, 5.00, and 10.00 μg/mL) for 48 h at 37 °C. Data are presented as the means ± SEM determined from five independent experiments. **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: CCK-8 Assay, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The averaged absorption spectra of WRL68 cells that were untreated (control group, black) and treated with 0.120 μg/mL vincristine (treated group, red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: The vector-normalized averaged second-derivative spectra of the WRL68 cells that were untreated (control, black) and treated with vincristine (red).

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the lipid region (3000–2800 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.925, R 2 Y = 0.704, Q 2 = 0.651). The x - and y -axes indicate the first component t1 and the first orthogonal component to1. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean ν as (CH 2 )/ν as (CH 3 ) and ν s (CH 2 )/ν s (CH 3 ) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells, **** P < 0.0001compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from WRL68 cells in the protein region (1800–1480 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.961, R 2 Y = 0.617, Q 2 = 0. 511). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean protein amide I/amide II (1670–1646/1563–1531) and α-helix/β-sheet (1670–1646/1644–1621) absorption band peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated cells. **** P < 0.0001 and *** P < 0.001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: (a) PCA score plot of the vector-normalized second-derivative spectra from the WRL68 cells in the fingerprint region (1480–900 cm –1 ). (b) Loading plots of PC1 and PC2 from the PCA model. (c) OPLS-DA score plot ( R 2 X = 0.597, R 2 Y = 0.873, Q 2 = 0. 754). The x - and y -axes indicate the first component t1 and the first orthogonal component to1, respectively. The y -axis displays the within-class variability (intraclass discrimination), and the x -axis displays the separation between the control and treated groups (interclass discrimination). The gray circle refers to the 95% confidence ellipse. (d) Mean nucleic acid ν as (PO 2 – )/lipid ester ν(C=O) (1269–1201/1754–1723), nucleic acid ν as (PO 2 – )/protein amide II (1269–1201/1563–1531), nucleic acid ν s (PO 2 – )/lipid ester ν(C=O) (1105–1070/1754–1723), nucleic acid ν s (PO 2 – )/protein amide II (1105–1070/1563–1531), and nucleic acid ν s (dianionic phosphate monoester)/lipid ester ν(C=O) (984–948/1754–1723) peak area ratio values measured from the second-derivative spectra of the control and vincristine-treated groups. **** P < 0.0001 compared with the control group.

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques: Plasmid Preparation, Control

Journal: ACS Omega

Article Title: Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

doi: 10.1021/acsomega.2c06622

Figure Lengend Snippet: Assignments of the Most Relevant IR Absorption Bands of WRL68 Cells, Together with the Related Vibrational mode 28 − 30

Article Snippet: WRL68 human embryonic liver cells (obtained from Procell Life Science & Technology Co., Ltd.) were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM, obtained from KeyGEN BioTECH) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO 2 saturation in a humidified atmosphere.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Evaluation of chemical constituents and in vitro antimicrobial, antioxidant and cytotoxicity potential of rhizome of Astilbe rivularis (Bodho-okhati), an indigenous medicinal plant from Eastern Himalayan region of India

doi: 10.1186/s12906-019-2621-6

Figure Lengend Snippet: Cytotoxicity effects of AR rhizome extract  against the Neuroblastema cell (SHSY5Y), Human Embryonic kidney cell (HEK- 293) and Liver cell line (WRL-68). Data represent the mean of three replicates

Article Snippet: Neuroblastoma (SHSY5Y), Human Embryonic Kidney (HEK-293) and Liver (WRL-68) cell lines were obtained from National Centre for Cell Science, Pune, India.

Techniques:

Journal: bioRxiv

Article Title: Anti-Tumor Potential of AEV01 in Glioma: In Vitro and Xenograft Model Studies

doi: 10.1101/2024.07.22.604571

Figure Lengend Snippet: I and II-Histopathological examination on the vital organs of HCC (Hep3B)-induced, AEV01-treated rats such as heart, lungs, pancreas, kidney and spleen were quantified at 40x magnifications by inverted microscope (b) quantitative data based on the cellularity index was obtained employing ImageJ. Data are represented in terms of mean ± SD, n=3.

Article Snippet: HEK-293T (Human embryonic kidney cell line) (Passage No: 10), U-87 MG human glioblastoma (GB) cell line (Passage Number: 58), HepG2 human liver cancer cell line (RRID: CVCL_GP63) (Passage Number: 58), Hep3B (Passage Number: 35) and WRL-68 liver senescence cells (Passage Number: 68) were procured from National Centre for Cell Sciences (NCCS) (Pune, India).

Techniques: Inverted Microscopy